Table 3. Oligonucleotide primers used for plasmid construction in this study.

Gene (Locus)	Description	Enzyme sites	Primer sequence
TWL (MGG__02916.6)	Deletion construct	-	5’-GGGGCTCCTGGAATAGATTGGGGT-3’
		-	5’-ACCAGCAAGCAGGAAGCGGACGCG-3’
		PstI	5’-GACTGTTCTGCAGGATGACATTGCATACAACGTCCAA-3’
		HindIII	5’-GAGTGTTAAGCTTATAGCGTCATGGTCTGGAATGAAC-3’
	GFP-tagging (N-	HindIII	5’-GAGTGTAAGCTTCCAGGTGGGTGCCAAGGGGAAGGAG-3’
	terminal) under RP27 promoter	-	5’-GACCCTTGTTGCATGAGGCCTGGG-3’
		KpnI	5’- GAGAGTGGTACCATGTCCAAGCCTGAGCTTTTGGG -3’
		-	5’- CCGTAGCTCTGgaattcGACACAG-3’
		BamHI	5’-GAGAGTGGATCCATAAATGTAGGTATTACCTGTA-3’
		NcoI	5’-GAGAGTGACCATGGTTTGAAGATTGGGTTCCTACGA-3’
		NcoI	5’-GAGAGTGACCATGGTGAGCAAGGGCGAGGAGCTGT-3’
		KpnI	5’-GAGAGTGAGGTACCCTTGTACAGCTCGTCCATGCCGAG-3’
	genomic DNA for promoter and N-	-	5’-ggggctcctggaatagattggggt-3’
	terminal 150 aa genomic DNA for N-terminal 120 aa	XmaI	5’-GAGTGACCCGGGAGCTCTGGAATTCGACACAGGCGG-3’
		KpnI	5’- GAGAGTGAGGTACCATGTCCAAGCCTGAGCTTTTGG-3’
		-	5’- GCCGCCTGTGTCGAATTCCAGA-3’
SNF1 (MGG_00803.6)	Deletion construct	EcoRI	5’-GTGTGAATTCGGTGTGCCCAAAGACTGCCTTG-3’
		KpnI	5’-GTGTGGTACCGGCGGATGGAGGGGATTGCTG-3’
		HindIII	5’-GTGTAAGCTTACCCAGGTCAATGCGATCGC-3’
		HindIII	5’-GTGTAAGCTTGCAACCGGAAGACTTGAGAG-3’
TFB5 (MGG_15089.6)	GFP-tagging (N-terminal) under RP27 promoter	KpnI	5’- GAGAGTGAGGTACCATGCCCAAGGCAGTCCATGGT -3’
		EcoRI	5’- GAGTGAGAATTCGCAAGAGCCCCGTGGCGGTGCAGG -3’