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. 2015 Jun 23;11(6):e1005311. doi: 10.1371/journal.pgen.1005311

Fig 1. Physical interaction between BBS proteins and IR.

Fig 1

A) Sedimentation analysis showing the β subunit of the IR in complex with the BBSome (corresponding to fraction 9). B-C) Reciprocal co-immunoprecipitation with GFP-tagged BBS17 and the β subunit of the IR in protein lysates from HEK293T cells. The interaction between the IRβ subunit and BBS17 can be detected whether IR antibody is used for immunoprecipitation (A) or GFP-BBS17 is used to precipitate IR (Ctrl line in C). Silencing the Bbs1 gene with shRNA disrupt the interaction between BBS17 and IRβ (right line in C).