Table 1. Microbes targeted for screening Osmia cornifrons.
| Target | PCR primers | Annealing temp (°C) | Alignment length (bp) | GenBank accession nos. |
|---|---|---|---|---|
| Ascosphaera spp.: internal transcribed spacer 1 (partial), 5.8S, internal transcribed spacer 2 (partial) | AscoAll-F, AscoAll-R [43] | 62 | 466 | KP340870–KP340896 |
| Aspergillus spp.: 28S rRNA subunit (partial) | AF4, AR1 [46] | 54 | 222 | KP340862–KP340869 |
| Microsporidia: small rRNA subunit (partial) | MicroF, 1492N [47] | 54 | N/A | N/A |
| Paenibacillus spp.: 16S rRNA subunit (partial) | AF1f, AF2 [48] | 58 | 146 | KP340861 |
| Trypanosomes: small rRNA subunit (partial) | TrypanF1, TrypanR1 [49] | 58 | N/A | N/A |
| Wolbachia spp.: cytochrome oxidase A (partial) | coxF1, coxR1 [50] | 54 | N/A | N/A |