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Fe3+-heme difference titrations with HO2core, HO2tail(C282A)R, HO2tail(C265A)R, and HO2solR. Difference titrations were performed in 50 mM Tris (pH 8.0) and 50 mM KCl at 20 °C under anaerobic conditions with (A) 2 μM HO2core, (B) 10 μM HO2tail(C282A)R, (C) 6 μM HO2tail(C265A)R, or (D) 5 μM HO2solR. Insets in panels A–C are plots of the respective data at the indicated wavelength with the solid lines representing the fits to the quadratic equation to obtain Kd values.
