Figure 1.

Genotype and phenotype of P66 integrin‐binding region mutants.

A. Nucleotide and amino acid sequence of WT, D205A,D207A and Del202–208 strains. Mutations in the nucleotide sequence that resulted in altered amino acids are in bold and italics for mutations at a.a. 205 and 207. Nucleotides removed from the coding region are represented as dashes for Del202–208.

B and C. Surface expression of P66 in various B orrelia burgdorferi strains (B: HB19 strains, C: B31‐A3 strains). The bacterial strains were incubated with varying concentrations of proteinase K (1, 10, 100 μg ml⁻¹) for 1 h. Untreated bacteria were used as a negative control. Bacterial lysates were run on a 10% SDS‐PAGE gel and transferred to an Immobilon membrane that was probed with antibodies to P66 and flagellin. Colorimetric methods were used for visualization. cp = p66 gene restored on a shuttle plasmid, cc = p66 gene restored to endogenous locus on chromosome.