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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Neurosci Res. 2015 Feb 24;93(8):1258–1266. doi: 10.1002/jnr.23576

Figure 4.

Figure 4

Inhibition of the association between GSK3β (p-Ser 9) and PP1/I2PP1 was detected during long-term treatment with shCDK5miR. a) Changes in the GSK3β and PP2A/I2PP2A protein levels were maintained after one year of CDK5 knockdown in 3xTgAD mice. Representative blots are shown. Tubulin was used as a loading control, and densitometry quantification was performed. n=3, *=p<0.05, **=p<0.01, ***=p<0.001. b) The I2PP1 and PP1 association was evaluated using Immunoprecipitation. This association suggests persistent PP1 inactivation, and changes in PP2A activity were not detected at one year post-injection. Quantification and representative blots are shown. Densitometry quantification was performed. n=5, *=p<0.05.