Skip to main content
. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Gastroenterology. 2015 Mar 18;149(1):211–222.e10. doi: 10.1053/j.gastro.2015.03.014

Figure 2. NRF2 activation inhibits expression of ITPR3.

Figure 2

(A) Immunoblots of cytosolic and nuclear fractions from NHC cells treated with DMSO or quercetin (25 μM) demonstrate that quercetin increases NRF2 expression, predominantly in the nucleus. Tubulin was used as a non-nuclear marker and lamin B was used as a nuclear marker to confirm purity of the cytosolic and nuclear fractions, respectively. (B) Confocal immunofluorescent staining of NHC cells demonstrates that treatment with quercetin increases NRF2 (red) and decreases ITPR3 expression (green). Nuclei were labeled with the nuclear marker TOPRO-3 (blue). Scale bar, 10 μm. (C) Relative mRNA expression of NRF2 is increased in NHC cells treated with quercetin. Values here and in subsequent panels are mean ± SD of results from triplicate experiments; *p<0.05. (D) mRNA (left) and protein (right) expression of ITPR3 are decreased by quercetin treatment. mRNA levels were normalized to β-actin. Results are presented as fold changes relative to DMSO-treated controls. Relative mRNA (left) and protein (right) expressions of (E) NRF2 and (F) ITPR3 in NHC cells transfected with scrambled or NRF2-shRNA shows that knockdown of NRF2 does not alter ITPR3 expression.