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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Gastroenterology. 2015 Mar 18;149(1):211–222.e10. doi: 10.1053/j.gastro.2015.03.014

Figure 3. The NRF2 activator, tert-butyl hydroquinone (t-BHQ), and NRF2 overexpression also decrease ITPR3 expression.

Figure 3

Relative protein expression of (A) NRF2 and (B) ITPR3 in NHC cells treated with t-BHQ (75 μM) in parallel with treatment of quercetin (25 μM) for 48 hours. Quantified protein expression was normalized to GAPDH. Results are expressed as fold change relative to solvent control (DMSO). *p<0.05; significantly different between indicated groups (n=4 independent experiments). Relative mRNA and protein expression of (C) NRF2, and targets of Nrf2 (D) GCLC, (E) HO-1, and (F) ITPR3 in mouse cholangiocytes transduced with either empty control GFP-adenovirus or adenovirus expressing NRF2. Data are mean ± SD compared to controls (n=3), *p<0.05. Ad, adenovirus.