Figure 2. Histochemical verification of microwire placement.
Representative coronal slices through the rat striatum. All slices were processed for anti-calbindin d28-k, which histochemically differentiates the NAc Shell and Core as well as the DLS from the dorsomedial and ventral striatum. All NAc Shell and DLS microwires were localized to calbindin d28k immunonegative regions. All NAc Core neurons were localized to calbindin d28k immunopositive regions. Arrow head in 1.28mm section shows an actual lesion left by a microwire tip.