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. Author manuscript; available in PMC: 2016 Jul 17.
Published in final edited form as: AIDS. 2015 Jul 17;29(11):1287–1296. doi: 10.1097/QAD.0000000000000698

Figure 1. Soluble CD40L circulates in plasma as monomers and multimers, which exert differential effects on DCs.

Figure 1

(A) sCD40L forms in plasma from 8 HIV-infected donors and 8 healthy donors were demonstrated by immunoblot analysis. (B) DC purity as measured by FACS, DCs were incubated with either sCD40L monomer (MONO), CD40L multimer (MULTI), as compared to control DC (NEG) overnight and (C) CD86 surface expression was evaluated by flow cytometry and (D) IL-6 and TNF-α production were measured in cell supernatant by cytokine bead array analysis. (E) DCs were incubated with either sCD40L monomer (MONO), CD40L multimer (MULTI) as compared to control DC (NEG) overnight and then were subsequently stimulated overnight with Poly-ICLC, and IL-12 production was measured in cell supernatants by cytokine bead array. Shown in (C), (D), and (E) are data for three donors with statistical analysis using two-tailed Student's t tests. *Represents a p-value < 0.05 and was considered significant.