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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Stem Cells. 2015 May 25;33(7):2320–2330. doi: 10.1002/stem.2048

Figure 4. Loss of Faap20 induces early erythroid progenitors and MEPs ablation after MMC treatment.

Figure 4

Faap20−/− or wild type mice at 6-8 weeks of age were injected with one dose 8mg/kg MMC to induce the bone marrow failure. PBS injected Faap20−/− or wild type mice were used as controls. Mice were analyzed 8 days after injection. (A) The early erythroid progenitors in Faap20−/− mice were depleted after MMC exposure. Freshly dissociated bone marrow cells were labeled with CD71 and Ter119 mAb, and subjected to flow cytometric analysis. The five regions (R1 to R5) within a flow cytometry scatter plot contain distinct cell types found in the different stages of erythrocyte differentiation were selected as indicated. There are predominantly proerythroblasts in region R1, basophilic erythroblasts in region R2, late basophilic and chromatophilic erythroblasts in region R3, orthochromatophilic erythroblasts in region R4, and reticulocytes and erythrocytes in R5. (B) The percentages of R2-R5 cells in the bone marrow were quantitated from the data shown in (A). (C) Flow cytometry analysis show early progenitor depletion in Faap20−/− mice treated with MMC. (D) Quantitation of CMPs, MEPs and GMPs in Lin-cKit+ (LK) compartment. Data represent summary of more than three mice of each genotype from two independent experiments. (E) Quantitation of CLPs. Data represent summary of more than three mice of each genotype from two independent experiments.