Figure 3.

Null- and preferred-side SAC-DSGC synapses display similar paired-pulse depression throughout development. A, Example traces from paired-pulse protocol. Top traces of each color show tail currents elicited from a null (red) and a preferred side (blue) P14 SAC at varying interstimulus time intervals (100, 250, 500, 1000, 1500 ms) in Ca²⁺-ACSF. Bottom traces show responses of their shared postsynaptic DSGC held at 0 mV. B, IPSCs evoked from preferred- and null-side SACs display the same kinetics. Peak normalized traces from A for 250, 1000, and 1500 ms interstimulus intervals. C, The average paired-pulse ratio for P13–P14 SAC-DSGC pairs decreases as external Ca²⁺ increases for the 250 ms interstimulus interval. All groups are significantly different from each other (one-way ANOVA, p = 2.2 × 10⁻⁹, df = 35; Tukey-Kramer post hoc, p < 0.05). D, Paired-pulse ratio at the beginning and end of the second postnatal week as a function of interstimulus interval. Data points for the 250 ms interstimulus interval recorded in the same DSGC are connected via dashed lines of the same color.