Figure 2.

Coexpression of TDP-43 with granulin 2 or 3 synergistically delays larval development. A, Models of Homo sapiens versus C. elegans progranulin protein and FLAG-tagged granulin constructs (TM domain, transmembrane domain). B, Anti-FLAG Western blot (top) and quantification (bottom) demonstrates granulin expression in indicated strains. No significant differences are found (n = 3 biological replicates; one-way ANOVA; error bars represent SD). C, Gran1::GFP, gran2::GFP and gran3::GFP are all localized in a subset of vesicles in intestinal cells. Representative images from each line are shown. Scale bar, 10 μm. Arrowheads indicate intestinal nuclei. D, All three granulins were efficiently secreted from their cells of origin and taken up by coelomocytes, which do not produce progranulin (Kao et al., 2011). Representative images of granulin::GFP in coelomocytes is shown. Scale bar, 10 μm. Arrows indicate posterior coelomocytes.