Figure 6.

Expression of TDP-43 with granulins 2 and 3 results in a constitutive egg-laying (Egl-C) phenotype. A, Animals were treated with 5 mg/ml serotonin hydrochloride (5HT) for 1 h and then eggs laid were quantified (n = 8 per strain). B, Animals were treated with M9 for 1 h and then eggs laid were quantified. Granulin; TDP-43 animals exhibited constitutive egg laying (n = 8 per strain). C, The percentage of early-stage eggs laid by Day 1 adults from each strain. Granulin; TDP-43 animals exhibited premature egg laying (data shown are the average of two trials, n ≥ 100 per trial). For all panels: ns, not significant; wt, wild-type; mut, mutant; *p < 0.05, **p < 0.01 by one-way ANOVA. Error bars represent SEM.