DA- and dop-3-dependent Swip mutants vt29 and vt33 harbor mutations in F53B1.6. A, Genomic localization of mutations in vt29 and vt33 and gene diagram of F53B1.6. Lines vt29 and vt33 harbor, respectively, nonsense and a nonconservative missense mutation in exon 10 of FB3B1.6. The tm5915 allele deletes 339 bp spanning F53B1.6 exons 2, 3, and 4 along with a 2 bp insertion. B, Manual Swip analyses of vt29, vt33, and tm5915 demonstrated that these lines exhibit DA synthesis (cat-2)- and DA receptor (dop-3)-dependent Swip. Assays were performed as described in the Materials and Methods. Data were analyzed using one-way ANOVA with multiple Bonferroni posttests where ****p < 0.0001 and error bars represent SEM. Asterisks indicate a comparison of double mutants back to the single mutant vt29, vt33, and tm5915, respectively. Loss of cat-2 and dop-3 alone does not result in a significant change in swimming.