Figure 3.
Expression of F53B1.6 in glial cells is sufficient to rescue Swip. Expression of a swip-10 genomic fragment significantly restores swimming behavior in vt29 (second bar) and vt33 (seventh bar) mutants. Similarly, expression of swip-10 cDNA under the control of a pan glial promoter ptr-10, and not in DA neurons, significantly rescues vt29, vt33, and tm5915. Mutation of histidine residues within the conserved canonical β-lactamase motif significantly reduces the ability of the pptr-10: swip-10 cDNA to rescue vt29. For all experiments, transgenic lines were generated and tested as described in the Materials and Methods. The promoter used to drive expression of the swip-10 gene, and swip-10 coding sequences (CDS; either genomic or cDNA) are noted beneath the bars. The basal Swip values from nontransgenic animals shown for swip-10(vt29), swip-10(vt33), and swip-10(tm5915) are representative averages obtained during these experiments and consistent with values presented in Figure 1. Values from at least three independent transgenic lines were pooled to generate the averages shown. Data were analyzed using an unpaired Student's t test comparing nontransgenic and transgenic progeny assayed in parallel. **p < 0.01, ***p < 0.001, ****p < 0.0001, ####p < 0.0001 from a t test between averages of transgenic progeny.