Mitochondrial respiratory chain protein levels determined in cytoplasmic skeletal muscle lysates before (black bars) and after (grey bars) 12 weeks of RET. I, II, III, IV and V represent complex I (NADH oxidase), complex II (succinate dehydrogenase), complex III (cytochrome bcoxidoreductase), complex IV (cytochrome C oxidase) and complex V (ATP synthase) of the respiratory chain are shown in Panel 4A. Representative blot images of the human heart mitochondria isolate internal control and a pre and post sample (loaded in duplicate) are shown in Panel 4B. Changes in RNA levels of COX18, COX4I1, MFN1, NAMPT, NDRG2, PGC1α, and TFAM pre and post RET are presented in Panel4C. *P<0.05.