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. 2014 Jul 16;9(1):71–83.

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Copyright© Iranian Society of Medical Entomology & Tehran University of Medical Sciences

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Fig. 9 — Diagnostic PCR-RFLP to identify G119S mutation in amplified region of ACE-1 (194 bp) in individuals of Anopheles stephensi. M: 50 bp ladder (Fermentas), Lane 1–6: temephos-resistant strain (Lane 1–2: 4^th generation of selected strain with temephos, Lane 3–4: 5^th generation of selected strain with temephos, Lane 5–6: 6^th generation of selected strain with temephos) CLUSTAL 2.1 multiple sequence alignment