Table 2.

List of primer used for molecular identification of An. culicifacies sibling species

Sequence no.	Primer	Sequence (5′-3′)	Tm
1	D3A	GAC CCG TCT TGA AAC ACG GA	67.3
2	D3B	TCG GAA GGA ACC AGC TAC TA	61.3
3	ACA	GCC GTC CCC ATA CAC TG	62.7
4	ACB	CCG TAA TCC CGT GAT AAC TT	60.2
5	ADF	CTA ATC GAT ATT TAT TAC AC	45.5
6	ADR	TTA CTC CTA AAG AAG GC	48.8
7	DF	TTA GAG TTT GAT TCT TAC	42.9
8	BCEF	AAA TTA TTT GAA CAG TAT TG	48.4
9	BCR	TTA TTT ATT GGT AAA ACA AC	48.6
10	CR	AGG AGT ATT AAT TTC GTC T	49.3
11	ER	GTA AGA ATC AAA TTC TAA G	45.1