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. 2015 Jun 24;5:54. doi: 10.3389/fcimb.2015.00054

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Copyright © 2015 Dabral, Jain-Gupta, Seleem, Sriranganathan and Vemulapalli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of RB51-specific (A) IgG, (B) IgG1, (C) IgG2a, (D) IgG2b, and (E) IgG3 antibodies in serum of mice vaccinated with live RB51, live recombinant RB51WbkA and saline-inoculated mice. Serum samples were collected at 3 and 6 weeks after vaccination, were diluted 1 in 100 and assayed for the presence of total antigen-specific antibodies by indirect ELISA. Results are shown as mean ± standard deviation (n = 4) of absorbance of the color developed. ^*Significantly different from the corresponding saline group at week 3 (P < 0.01). ^**Significantly different from the corresponding saline group at week 6 (P < 0.01).