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. 2015 Jun 24;5:11497. doi: 10.1038/srep11497

Figure 2. Non-transcriptional p53 is involved in Bax mediated apoptosis.

Figure 2

(A) Bax mitochondrial translocation in heat stress treated cells is inhibited by PFT inhibition of p53. Bax protein levels in cytosol and mitochondria were analyzed using western blot(cropped). COX IV, mitochondrial loading control. (B) Bax conformational change is diminished by PFT inhibition of p53. Bax (6A7) with conformational changes was specifically detected by immunoprecipitation(cropped) with an anti-Bax 6A7 monoclonal antibody. (C–E) Non-transcriptional p53 apoptosis analysis in H1299 cells overexpressing p53NLS. (C) Bax protein levels in cytosol and mitochondria were analyzed using western blot(cropped). COX IV, mitochondrial loading control. (D) Bax conformational change is diminished by PFT inhibition of p53. Bax (6A7) with conformational changes was specifically detected by immunoprecipitation(cropped) with an anti-Bax 6A7 monoclonal antibody. (E) apoptosis was analyzed by flow cytometry using an Annexin V-FITC/PI staining kit. Data are presented as means ± SD of three separate experiments. *p < 0.05 as compared to control; #p < 0.05 as compared to heat stress (HS) alone.