Figure 5. Heat stress induces the production of ROS in HUVEC cells.

(A) Heat stress treatment leads to increase in ROS generation in HUVEC cells. Laser scanning confocal microscope images of cells stained with DHE were shown (red). The images shown are representative of the three independent image acquisitions. (B) Quantitative analysis of the increase in ROS generation upon heat stress treatment in HUVEC cells. The amounts of ROS were analyzed by flow cytometry upon staining with fluorescent dye DHE. X/XO (0.1 mM X + 0.01 U XO) was used as a positive control. Data are presented as mean ± SD for three independent experiments, *P < 0.05 compared with control group (37 °C).