Figure 7. Heat stress induced ROS production is the upstream signal required for the triggering of downstream apoptosis initiating events.

(A) ROS reduction by MnTBAP pretreatment suppresses p53 mitochondrial translocation in heat stress exposed cells. Protein levels of p53 in cytosol and mitochondria were measured by western blot(cropped). COX IV, mitochondrial loading control. (B) ROS reduction by MnTBAP diminishes MPTP opening in heat stress exposed cells. MPTP opening was assessed by staining with calcein-AM and COCl₂. (C,D) ROS reduction by MnTBAP diminishes Bax conformational change and mitochondrial translocation in heat stressed exposed cells. Bax tranlocation and conformation change were measured by western blot and immunoprecipitation, respectively.