TABLE 1.
Bacterial strains
| Strain | Genotype | How constructed | Source or reference |
|---|---|---|---|
| Mycobacterium bovis BCG | |||
| mc26450 | Parental, Danish strain | AERAS | |
| mc26452 | BCG Danish ΔleuCD::γδ-res hyg sacB γδ-res | ST with phAESP22 (42) | This study |
| mc26454 | BCG Danish ΔleuCD::γδ-res | Unmarking with phAE201 | This study |
| mc26585 | BCG Danish ΔleuCD::γδ-res (pYUB2115) | Transformation with pYUB2115 | This study |
| SL524 | BCG Danish ΔleuCD::γδ-res (pSL701) | Transformation with pSL701 | This study |
| SL525 | BCG Danish ΔleuCD::γδ-res (pSL720) | Transformation with pSL718 | This study |
| SL547 | BCG Danish ΔleuCD::γδ-res (pSL509) | Transformation with pSL509 | This study |
| E. coli | |||
| DH5α | Chemically competent | Life Technologies | |
| JW0070-1 | F− Δ(araD-araB)567 ΔleuD778::kan ΔlacZ4787(::rrnB-3) λ− rph-1 Δ(rhaD-rhaB)568 hsdR514 | 43 |