TABLE 2.
Plasmid constructs and phages
| Plasmid construct/phage | Description | Source or reference |
|---|---|---|
| Plasmids | ||
| pYUB2115 | oriM, oriE, Paph, leuCDM. smegmatis | This study |
| pSL701 | PgroEL2, lpqH signal sequence (aaa 1–46)-HIV gp120-lpqH (aa 71–158) fusion | This study |
| pSL718 | PgroEL2, fbpB signal sequence (aa 1–41)-HIV gp120-lpqH (aa 71–158) fusion | This study |
| pSL720 | PgroEL2, fbpB signal sequence (aa 1–41)-HIV gp120-lpqH (aa 71–145) fusion | This study |
| pSL509 | PgroEL2, fbpB signal sequence (aa 1–41)-SIV gagmac239 fused to hemagglutinin | This study |
| Phages | ||
| phAESP22 | M. tuberculosis leuCD::hyg; used to construct mc26452 (Table 1) | This study, 42 |
| phAE201 | Unmarking phage (γδ resolvase); used to construct mc26454 (Table 1) | This study |
a
aa, amino acid.