TABLE 3.

Quality control analysis of accession lot physical properties

Lot	Strain	Clumpinga		Uniform biomassb				Liquid culturec		Purity resultd	Mean CFU/OD (×108)e
		Score	Result	Avg	SD	CoV (%)	Result	Day OD >0.2	Result		Avg CFU	CoV (%)	CFU/OD	Result
036	SL521	15.60	Pass	1.23	0.01	1	Pass	1.00	Pass	Pass	4.78	7	3.90	Pass
039	SL524	19.00	Pass	6.50	0.46	7	Pass	0.85	Pass	Pass	18.50	17	3.25	Pass
040	SL525	14.00	Pass	5.70	0.10	2	Pass	1.10	Pass	Pass	9.67	48	1.49	Pass
062	SL547	14.00	Pass	5.93	0.15	3	Pass	0.85	Pass	Pass	18.70	24	3.15	Pass

^aParticle sizes of rBCGD ΔleuCD clumps visualized by microscopy were assigned a clumping score. A passing score was <40.

^bConsistency of optical density between three vials was assessed by OD₆₀₀ covariance (CoV). The target OD₆₀₀ for lot 036 was 1 and the target for lots 039, 040, and 062 was 5. A passing covariance was <15%.

^cRecovery of thawed accession lot material in liquid culture was examined by daily monitoring of the optical density. A passing lot achieved OD₆₀₀ of >0.2 in <7 days of growth.

^dThe accession lot material was streaked on chocolate agar, and plates were observed for contaminating organisms. A passing lot yielded no contaminating colonies after 28 days of incubation.

^eThe accession lot material was diluted and plated to measure the ratio between the number of CFU and optical density. A passing lot possessed a CFU/OD ratio of >0.1.