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. 2015 Jun 23;22(7):711–725. doi: 10.1128/CVI.00763-14

TABLE 1.

C. difficile toxin A and toxin B recombinant fragments used for domain mapping

Toxin Domain Amino acids Fragment name
A C terminus of GST, N terminus of proteasea 300–660 TcdA300–660
C terminus of protease, N terminus of translocation domain 660–1100 TcdA660–1100
B GTD 10–520 TcdB10–520
Protease 510–1110 TcdB510–1110
Translocation domain of N terminus 1110–1530 TcdB1110–1530
Translocation domain of C terminus 1530–1750 TcdB1530–1750
CTD 1750–2360 TcdB1750–2360
a

GST, glutathione S-transferase.