Figure 5.

Characterization of chromosome instability (CIN) gene dosage sensitivity. (A) A bar plot shows CIN rate of nine strains each having heterozygous deletion of one icCIN gene (dark gray bar). These are thus two-way dosage-sensitive genes. Data are shown as mean ± SEM (n = 8). (B) A table displaying core complex involvement of six two-way dosage-sensitive CIN genes. (C) Bar plots showing the CIN rate of haploid strains with different copy number of genetically integrated NPL3. WT control has one native copy of NPL3. Data are shown as mean ± SEM, n = 8. P value was calculated from t-test. ****P < 0.0001. (D) Bar plots comparing the CIN rate of strains containing a blank centromeric plasmid, NPL3 wild-type centromeric plasmid, and NPL3 point mutant centromeric plasmid. Top panel indicates the position of the point mutation in the second RNA Recognition Motif (RRM2) of Npl3p. N (N terminus); C (C terminus). Mean ± SEM, n = 8. P value was calculated from t-test. **P < 0.01. (E) Bar plots showing the CIN rate of haploid strains with different copy number of genetically integrated MCD1. Data are shown as mean ± SEM, n = 8. P value was calculated from t-test. ****P < 0.0001. (F) Bar plots comparing CIN rate of strains containing two blank centromeric plasmids (Empty Plasmids Control), MCD1 centromeric plasmid plus one blank centromeric plasmid (MCD1), ESP1 centromeric plasmid plus one blank centromeric plasmid (ESP1), and MCD1 centromeric plasmid plus ESP1 centromeric plasmid (MCD1 + ESP1). Mean ± SEM, n = 8. P value was calculated from t-test. ***P < 0.001. (G) Bar plots comparing CIN rate of strains containing two blank centromeric plasmids (Empty Plasmids Control), MAD1 centromeric plasmid plus one blank centromeric plasmid (MAD1), MAD2 centromeric plasmid plus one blank centromeric plasmid (MAD2), and MAD1 centromeric plasmid plus MAD2 centromeric plasmid (MAD1 + MAD2). Mean ± SEM, n = 8. P value was calculated from t-test. ****P < 0.0001.