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. 2015 Apr 7;5(6):1081–1094. doi: 10.1534/g3.114.015933

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Copyright © 2015 Pearce et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Degradation assays for HCH isomers. Degradation of α-HCH, β-HCH, γ-HCH, and δ-HCH (left-hand axis) by UT26, LL01, LL02, and LL03 and relative quantification of metabolites by peak area (right-hand axis). Time postinoculation is shown on the bottom axis and the timescale for the γ-HCH assay has been adjusted to observe both the very fast (less than 1 hr) and very slow (96 hr) degradation of γ-HCH by different strains. Values are the mean of three biological replicates, with standard deviations. Identity of metabolites was confirmed by comparison to authentic standards or to metabolites produced with purified UT26 LinA or LinB enzymes. HCH, hexachlorocyclohexane; PCCH, pentachlorocyclohexene; PCHL, pentachlorocyclohexanol; TCB, trichlorobenzene.