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. 2015 Apr 14;5(6):1233–1245. doi: 10.1534/g3.115.017681

Figure 2.

Figure 2

Analysis of sexual development, vegetative growth, and stress resistance in Δchs7 strains. (A) Sexual development of the wild-type and four Δchs7 strains after 7 d on complete medium (BMM) and minimal medium (SWG). All strains form perithecia and ascus rosettes (right column), and eject ascospores toward the lids of the Petri dishes (spores on lids shown in small boxes in right column). (B) Vegetative growth was analyzed on BMM in race tubes over 10 d, error bars give SDs of three independent biological replicates. (C) Stress resistance was analyzed on SWG in Petri dishes with the indicated stress-inducing agents at the following concentrations: dithiothreitol (DTT) 0.04%, sodium dodecyl sulfate (SDS) 0.1%, KCl 0.6 M, glucose 0.6 M. The wild type was used as reference for the control. For each stress-inducing condition, the growth on the control plates for the corresponding strain was used as reference. Error bars indicate SDs for two independent biological replicates, each with three technical replicates.