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. 2015 Apr 14;5(6):1233–1245. doi: 10.1534/g3.115.017681

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Copyright © 2015 Traeger and Nowrousian

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sexual development in the Δsec22 mutant and complemented strains. Strain SEC22-GFP-NA expresses sec22 under its own promoter and terminator regions, whereas in SEC22-GFP-OE sec22 is under control of the strong, constitutive A. nidulans gpd promoter. Fruiting body formation was analyzed after 7 d on complete medium (BMM) and minimal medium (SWG). Ascus rosettes from perithecia grown on BMM medium were analyzed after 7−9 d. Ascospores from the Δsec22 strain show different degrees of melanization within one ascus (blue asterisks). Ascospores that were ejected toward the lid of the plate from strains grown on BMM were analyzed after 7−9 d (right column).