Fig. 6.

P-selectin binding is conserved within the wider MSP7 family across different Plasmodium species. P-selectin-MSP7 binding assays using AVEXIS were used to determine the conservation of binding within the MSP7 orthologues and paralogues of P. falciparum (a), P. vivax (b), and P. berghei (c). a P-selectin bound to PfMSRP2 and 5, but not PfMSRP1, 3 or 4. b Detectable P-selectin binding was observed with PvMSP7-6, but not PvMSP7-1 or −9. P. vivax MSP7s are labelled according to their arrangement on chromosome: 1 = PVX_082700, 6 = PVX_082675, and 9 = PVX_082655. c Both PbMSRP1 and 2 bound both human and mouse P-selectin baits. Bars represent mean + SD, n =3. A representative experiment from at least two independent experiments is shown in each case. In each experiment the positive controls (+) were PfMSP7 preys screened against OX68 bait and negative controls (−) were PfMSP7 preys screened against the Cd4 tag bait