Fig. 3.

Replication kinetics and thermal stability of FmoPV. (A) Replication kinetics of FmoPV in CRFK cells. CRFK cells were inoculated with FmoPV at a MOI of 1 TCID₅₀. After incubation at 37°C for 3 hr, the infected CRFK cells were washed three times and cultured in growth medium. The culture supernatants were collected at the indicated times and then titrated. (B) Thermal stability of FmoPV at 4°C, 25°C or 37°C. The data also show the comparison of virus titers with directly infected and freeze-and-thaw infected samples. The data are the means ± standard deviations of three experiments. Inactivation of FmoPV by heat treatment at 60°C (C) and 70°C (D). Stock viruses were incubated for indicated time up to 30 min in water baths with temperatures of 60°C and 70°C, respectively. The data are the means ± standard deviations of three experiments.