Table 1. Summary of the contribution of DiI-labeled endoderm cells within a small area of the lateral-most domain of the AIP lip (at the first intersomite junction level in 9–11 somite embryos) to the gallbladder primordium after whole embryo cultures for 24–48 hra).
Marking side | Total number of embryos analyzed |
Number of embryos with labeled cells in | |||
---|---|---|---|---|---|
Gallbladder | Liver bud | Gutb) | Visceral endoderm | ||
Right | 25 | 25 | 1 | 3 | 8 |
Left | 7 | 3 | 1 | 4 | 4 |
a) The DiI-labeled cells in the gallbladder primordium were determined by anti-SOX17 immunostaining using serial frozen sections of the cultured embryos. In contrast, the DiI-labeled cells in the liver bud or other regions were histologically estimated. b) Some DiI-labeled cells were found to be located in the posterior gut region including presumptive ventral pancreatic bud.