Fig. 2.

Heterogeneous AP invasion-efficacy in oblique dendrites. (a) Layer 5 pyramidal neuron filled with JPW-3028. (b) Optical recordings of backpropagating APs obtained from two oblique branches (ROI 1 and ROI 2) are aligned with a whole-cell recording from the cell body (ROI 0). Horizontal dashed line marks the amplitude of the first AP at each sister branch. (c) Scatter plot of AP half-widths obtained from 27 oblique dendrites belonging to 20 pyramidal cells. Dashed horizontal line is the mean AP half-width measured in proximal basal dendrites as shown in Fig. 1(c). Gray rectangle marks two to five standard deviations above the mean AP half-width measured in proximal basal dendrites as shown in Fig. 1(c). (d) Same as in (c) except AP amplitude ratios (third/first) are plotted. The mean and two to five standard deviations are based on proximal basal dendrites (Fig. 1). $n$—number of cells; $N$—number of dendrites.