Figure 4.

Silencing of Nf-κb in KCs in ob/ob mice improves glucose tolerance without affecting liver steatosis. A) Protocol of 5- and 15-day GeRP treatment. 8-week-old mice were used for the 5-day treatment and 7-week-old mice were used for the 15-day treatment. B) Hematoxylin-eosin liver sections from mice treated with SCR- or NF-κB-GeRPs and (C) GTT (1 g/kg) were performed on mice treated with SCR- or NF-κB-GeRPs after withholding food for 6 hours. D) F.C. of Akt phosphorylation by insulin measured by densitometry of pSer473-Akt normalized to total Akt (ob/ob, n = 9–10; WT, n = 3). E) mRNA levels of gluconeogenic genes in liver from mice treated for 15 days with SCR- or NF-κB-GeRPs (n = 5). Results are presented as mean of fold change (F.C.) normalized to SCR-GeRPs–treated mice ± sem. *P < 0.05; **P < 0.01. The statistical significance was analyzed by t test or ANOVA followed by Tukey posttest.