Figure 3.

IMQ-induced chemokines expression in epidermis LyzM-Cre KO mice is not inhibited. The 5% IMQ cream was applied daily to control (C) or LyzM-Cre STIM1^fl/fl mice (KO). After 3 days, RNA was isolated from back skin, and a quantitative RT-PCR analysis was performed for each indicated gene: CXCL1 (A), CXCL2 (B), IL-17a (C), IL-22 (D), and IL-23a (E). Relative quantification determined with reference to 18S rRNA and normalized to control cream-treated control mice. Bar graphs show mean values ± sem of 6 mice per group. *Significant difference of STIM1^fl/fl KO group compared with control (C) groups (P < 0.01) based on 1-way ANOVA followed by Bonferroni’s post hoc test.