Figure 2.

Epithelial cell proliferation is measured between the groups using percentage positive BrdU and PCNA staining. A) Percentage positive BrdU IEC per crypt (mice were injected with BrdU 24 hours before the mice were killed). B) PCNA percentage positive IEC per crypt. C) Representative images of PCNA staining for each study group. Results of (A) and (B) are expressed as means ± sd of epithelial cell counts from 10 well-oriented crypt–villus complexes from each mouse, n = 6/group. *P < 0.05.