TABLE 1.
RT-PCR primer sequences
| Mouse gene | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
| Bcl-2 | ATGCCTTTGTGGAACTATATGGC | GGTATGCACCCAGAGTGATGC |
| Bax | TGAAGACAGGGGCCTTTTTG | AATTCGCCGGAGACACTCG |
| Fas | GGTGGAAACAAACTGCACCC | ACAGTGTTCACAGCCAGGAG |
| FasL | ACCGCTCTGATCTCTGGAGT | GGCTGGTTGTTGCAAGACTG |
| TNF-α | CCGGGAGAAGAGGGATAGCTTGG | TCGGACAGTCACTCACCAAGT |
| IFN-γ | ATGAACGCTACACACTGCATC | CCATCCTTTTGCCAGTTCCTC |
| TLR-2 | ACAATAGAGGGAGACGCCTT | AGTGTCTGGTAAGGATTTCCCAT |
| TLR-5 | CTGCAACTGTGAACTTAGCA | ACTTTAGGGACCGCAT |
| TLR-7 | TGTCTCTTCCGTGTCCACAT | GGATGATCCTGGCCTATCTC |
| TLR-9 | CTGTACCAGGAGGGACAAGG | CAGTTTGTCAGAGGGAGCCT |
| IL-6 | TCTCTGGGAAATCGTGGAAA | GATGGTCTTGGTCCTTAGCC |
| IL-10 | ACCTGGTAGAAGTGATGCCCCCAGGCA | CTATGCAGTTGATGAAGATGTCAAA |
| EGF | TTCTCACAAGGAAAGAGCATCTC | GTCCTGTCCCGTTAAGGAAAAC |
| EGFR | GCATCATGGGAGAGAACAACA | TCAGGAACCATTACTCCATAGGT |
| Amphiregulin | GGTCTTAGGCTCAGGCCATTA | CGCTTATGGTGGAAACCTCTC |
| HB-EGF | CGGGGAGTGCAGATACCTG | TTCTCCACTGGTAGAGTCAGC |
| Epiregulin | CTGCCTCTTGGGTCTTGACG | GCGGTACAGTTATCCTCGGATTC |
| β-Actin | ATGGAGCCGGACAGAAAAGC | CTTGCCACTCAGGGAAGGA |
| 18S ribosomal RNA | ATCGACACTTCGAACGCACT | CACACGTCTGAACTTCGGGA |