Figure 5.

TRAIL does not alter Akt or NF-κB signaling. A) SGBS preadipocytes were stimulated with growth medium alone or with TRAIL. At the indicated time points, proteins were isolated and subjected to Western blot analysis to assess the activation state of the Akt and NF-κB pathways. β-actin was used as a loading control. “C” indicates a positive control. One representative of 3 independent experiments performed is shown. B, C) SGBS preadipocytes were stimulated with growth medium alone or with either TRAIL or TNF-α. B) At the indicated time points, NF-κB p65 immunofluorescence staining was performed. One representative of 3 independent experiments performed is shown. Scale bars, 100 µm. C) At the indicated time points, nuclear proteins were isolated and subjected to EMSA analysis to assess the transcriptional activity of NF-κB. “P” indicates a control for the probe alone. Asterisk indicates nonspecific signals. One representative of 3 independent experiments performed is shown.