Figure 4.

Expression levels and fold changes of selected functions and pathways. (A) Heatmap of all differentially expressed genes involved in targeted functions/pathways. Log₂ (fold change against the replete treatment) in both P-limited and N-limited treatments were plotted for genes encoding chlorophyll A-B binding proteins (B), light harvesting proteins (C), ribosomal proteins (D), lysosomal proteins (E), and TCA cycle proteins (F). For each category, a dot represents a single gene; bar represent mean ± SD of the Log₂ fold change. For each category and each treatment, t-tests were carried out to test whether the Log₂ fold change values were significantly different from 0, i.e., whether these genes are differentially expressed compared to the replete treatment, collectively. Different p-values are represented by different numbers of stars. For a list of these genes and their read counts and FPKM values, see Table S1.