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. 2015 Jun 24;6:631. doi: 10.3389/fmicb.2015.00631

Table 1.

Culture conditions for Prymnesium parvum.

Culture Media Nutrients Temp L:D cycle Irradiance
1 L1, -silica, 18ppt* 882 μM NO3
36.2 μM PO4−3
18°C 12:12 300 μE m−2 s−1
2 L1, -silica, N/100,18ppt 8.82 μM NO3 18°C 12:12 300 μE m−2 s−1
3 L1, -silica, P/100, 18ppt 0.362 μM PO4−3 18°C 12:12 300 μE m−2 s−1

Guillard and Hargraves (1993) or https://ncma.bigelow.org.

*

Salinity is indicated as parts per thousand (ppt).

Illumination was provided by Philips F20T12CW bulbs and measured using a QSL-100 sensor with QSP-170 deckbox (Biospherical Instruments, Inc.).