Response matrices from high-throughput all-optical circuit mapping of the barrel cortex. (a) Averaged compound IPSP input responses of control slices (), showing the strength of compound IPSP inputs from all columns and layers for all columns and layers. Responses elicited in various layers (, 4, or 5) of different columns (A to D) were arranged along the -axis and the region of photostimulation arranged along the -axis as a response matrix. The strength of compound IPSP responses are color-coded in grayscale as indicated in the scale bar. (b) Averaged compound IPSP input responses of deprived slices (), showing the strength of compound IPSP inputs from all columns and layers to all columns and layers. The pattern of responses is similar to the controls and responses were not significantly different in each region (two sample t tests, ).