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. 2015 May 19;6:7090. doi: 10.1038/ncomms8090

Figure 5. Induction of ISGs depends on both type I and III IFNs in vivo.

Figure 5

(a) B6.A2G-Mx1 wild type (WT), IFNAR KO, IFNLR KO and IFNAR/IFNLR DKO mice were treated with 25 μg anti-CD3 or isotype control (ITC) intra-peritoneally, and intestinal tissue sections prepared after 3 h. Induction of mRNA of the indicated ISGs was measured by qRT–PCR in wt, IFNAR KO, IFNLR KO and IFNAR/IFNLR DKO mice in small intestinal tissue, and expression relative to TBP is shown. Each data point represents an individual mouse. (b) Tissue sections from anti-CD3 i.p. injected B6.A2G-Mx1 wt and IFNAR/IFNLR DKO mice were prepared at 0 h and 12 h after injection. Paraffin-embedded sections were stained for Mx1 protein (intra-nuclear, green), CD3 (red) and nuclei (4,6-diamidino-2-phenylindole, blue). Pictures are representative of three mice for each condition. Scale bar, 20 μm. Also shown are micrographs at higher magnifications (High mag) taken at 12 h p.i., showing the nuclear speckle-like localization of Mx1 in the wt mouse gut, but not in the IFNAR/IFNLR DKO mouse gut. See also Supplementary Fig. 5.