Figure 5. Neutrophils augment lymphocyte proliferation in draining lymph nodes.

(a) C57BL/6 mice were treated with 50 μg of anti-Ly6G or isotype control antibody one day prior and at the time of intradermal injection of S. aureus, and proliferation was assessed by BrdU incorporation 72 h later. Percentage of CD3⁺BrdU⁺ cells is shown. (b) Percentage of B220⁺BrdU⁺ cells is shown after mice were treated as in a. (c) Percentage of BrdU⁺CD4⁺ and CD8⁺ T cells in draining and non-draining lymph nodes 3 days after injection of S. aureus into C57BL/6 mice treated with anti-Ly6G or isotype control antibody as in a. Means+s.d. are shown, three independent experiments with at least three mice per group were pooled. Data were analysed using an unpaired student's t-test (a,b) or a one-way analysis of variance with a Bonferroni correction for multiple comparisons (c) **P≤0.01; ****P≤0.0001; NS, not significant.