Fig. 4.

Co-expression of TDP-43^WT and TDP-43^Q331K in mice results in a loss of motor neurons from the anterior horn of the lumbar spinal cord. a–d Cresyl violet staining of motor neurons in the lumbar anterior horn of young NTg (a), TDP-43^WT (b), TDP-43^Q331K (c) and TDP-43^WTxQ331K (d) mice, showing a dearth of neurons present only in TDP-43^WTxQ331K animals (scale bar: 50 μm). e Cell counting of motor neurons in the lumbar anterior horn showed a significant loss of approximately 70 % of motor neurons in TDP-43^WTxQ331K mice compared with age matched non-transgenic and single transgenic animals. (** p < 0.001 vs all other age-matched genotypes)