Fig. 6.

Co-expression of TDP-43^WT and TDP-43^Q331K in mice results in a loss of pyramidal neurons from the layer V region of the motor and somatosensory cortex. (a-d) Cresyl violet staining of cells in the layer V region of the cortex of young NTg (a), TDP-43^WT (b), TDP-43^Q331K (c) and TDP-43^WTxQ331K (d) mice, showing a dearth of large pyramidal neurons in TDP-43^WTxQ331K animals, together with a mild reduction in TDP-43^Q331K mice. e Cell counting of large pyramidal cells in the cortex showed a significant loss of approximately 28 % of large pyramidal neurons in TDP-43^WTxQ331K mice compared with age matched non-transgenic and TDP-43^WT animals. Young TDP-43^Q331K single transgenic animals showed a mild, non-significant loss of approximately 12 % of pyramidal cells. (* p < 0.05 vs age-matched NTg and TDP-43^WT animals)