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Hydrocortisone (HC) induced a shift in the response of TLR4 to LPS in mouse fetal colonic organ culture. C57BL/6J fetal and adult mouse colonic tissues were incubated with LPS for 45 minutes and for 6 hours and IL-6 levels measured in the supernatant (a) E 18.5, (b) E18.5 from a mother treated with HC, and (c) 12 week adult mouse colonic tissues. The mean and SD are from triplicate wells, and are representative of three separate experiments, *p<0.05, **p<0.01vs.0 minute.
