Figure 5.
DAF-16 overexpression recovers lifespan reduction resulting from djr-1.2 deletion upon Mn exposure (50mM MnCl2, 30min). Overexpression of DAF-16::GFP restored lifespan in djr-1.2(tm1346) deletion animals. DAF-16::GFP alone and DAF-16::GFP; djr-1.2(tm1346) worms show increased lifespan compared with N2 worms both in the absence of Mn exposure (p<0.05 and p<0.05, respectively) and in the presence of Mn exposure (p<0.05 and p<0.01, respectively). No statistically significant differences were noted between same strains with or without Mn exposure (See Table 2 for all p values). Lifespans were compared with the log-rank test (n=30).