Figure 3.

CD spectra of dark-equilibrated and light-irradiated 10b-azob samples (∼33 μM in a 20/80 trifluoroethanol/water solution), as indicated. The light-irradiated sample was prepared as described in the caption of Figure 2. Inset: CD T-melt of the light-irradiated 10b-azob sample, monitored at 222 nm. The solid line is a fit of the data to a two-state model using the same thermodynamic parameters determined for the wild-type Trp-cage 10b.⁴⁰